1v03
From Proteopedia
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| - | [[Image:1v03.gif|left|200px]]<br /><applet load="1v03" size=" | + | [[Image:1v03.gif|left|200px]]<br /><applet load="1v03" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1v03, resolution 2.00Å" /> | caption="1v03, resolution 2.00Å" /> | ||
'''CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1'''<br /> | '''CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1V03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor] with BGC, IPH and CCN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] Known structural/functional Site: <scene name='pdbsite=AC1:Iph Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1V03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor] with <scene name='pdbligand=BGC:'>BGC</scene>, <scene name='pdbligand=IPH:'>IPH</scene> and <scene name='pdbligand=CCN:'>CCN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] Known structural/functional Site: <scene name='pdbsite=AC1:Iph+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V03 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: pest defense]] | [[Category: pest defense]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:13:14 2008'' |
Revision as of 08:13, 3 February 2008
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CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1
Overview
Plant beta-glucosidases play a crucial role in defense against pests. They, cleave, with variable specificity, beta-glucosides to release toxic, aglycone moieties. The Sorghum bicolor beta-glucosidase isoenzyme Dhr1 has, a strict specificity for its natural substrate dhurrin, (p-hydroxy-(S)-mandelonitrile-beta-D-glucoside), whereas its close, homolog, the maize beta-glucosidase isoenzyme Glu1, which shares 72%, sequence identity, hydrolyzes a broad spectrum of substrates in addition, to its natural substrate, 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxaxin-3-one., Structural data from enzyme.substrate complexes of Dhr1 show that the mode, of aglycone binding differs from that previously observed in the, homologous maize enzyme. Specifically, the data suggest that Asn(259), Phe(261), and Ser(462), located in the aglycone-binding site of S. bicolor, Dhr1, are crucial for aglycone recognition and binding. The tight binding, of the aglycone moiety of dhurrin promotes the stabilization of the, reaction intermediate in which the glycone moiety is in a deformed (1)S(3), conformation within the glycone-binding site, ready for nucleophilic, attack to occur. Compared with the broad specificity maize, beta-glucosidase, this different binding mode explains the narrow, specificity of sorghum dhurrinase-1.
About this Structure
1V03 is a Single protein structure of sequence from Sorghum bicolor with , and as ligands. Active as Beta-glucosidase, with EC number 3.2.1.21 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:15148317
Page seeded by OCA on Sun Feb 3 10:13:14 2008
Categories: Beta-glucosidase | Single protein | Sorghum bicolor | Bevan, D.R. | Czjzek, M. | Esen, A. | Henrissat, B. | Moriniere, J. | Verdoucq, L. | BGC | CCN | IPH | Dhurrin hydrolysis | Family gh1 | Pest defense
