1k8p
From Proteopedia
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[[Image:1k8p.gif|left|200px]] | [[Image:1k8p.gif|left|200px]] | ||
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'''Structure of the Human G-quadruplex reveals a novel topology''' | '''Structure of the Human G-quadruplex reveals a novel topology''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8P OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of parallel quadruplexes from human telomeric DNA., Parkinson GN, Lee MP, Neidle S, Nature. 2002 Jun 20;417(6891):876-80. Epub 2002 May 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12050675 12050675] | Crystal structure of parallel quadruplexes from human telomeric DNA., Parkinson GN, Lee MP, Neidle S, Nature. 2002 Jun 20;417(6891):876-80. Epub 2002 May 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12050675 12050675] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Lee, M P.H.]] | [[Category: Lee, M P.H.]] | ||
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: Parkinson, G N.]] | [[Category: Parkinson, G N.]] | ||
| - | [[Category: | + | [[Category: Dimeric quadruplex]] |
| - | [[Category: | + | [[Category: Double chain reversal loop]] |
| - | [[Category: | + | [[Category: Human telomere sequence]] |
| - | + | [[Category: Parallel stranded]] | |
| - | [[Category: | + | [[Category: Quadruplex dna]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:26:30 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:26, 2 May 2008
Structure of the Human G-quadruplex reveals a novel topology
Overview
Telomeric ends of chromosomes, which comprise noncoding repeat sequences of guanine-rich DNA, are fundamental in protecting the cell from recombination and degradation. Disruption of telomere maintenance leads to eventual cell death, which can be exploited for therapeutic intervention in cancer. Telomeric DNA sequences can form four-stranded (quadruplex) structures, which may be involved in the structure of telomere ends. Here we describe the crystal structure of a quadruplex formed from four consecutive human telomeric DNA repeats and grown at a K(+) concentration that approximates its intracellular concentration. K(+) ions are observed in the structure. The folding and appearance of the DNA in this intramolecular quadruplex is fundamentally different from the published Na(+)-containing quadruplex structures. All four DNA strands are parallel, with the three linking trinucleotide loops positioned on the exterior of the quadruplex core, in a propeller-like arrangement. The adenine in each TTA linking trinucleotide loop is swung back so that it intercalates between the two thymines. This DNA structure suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomere-associated proteins.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of parallel quadruplexes from human telomeric DNA., Parkinson GN, Lee MP, Neidle S, Nature. 2002 Jun 20;417(6891):876-80. Epub 2002 May 26. PMID:12050675 Page seeded by OCA on Fri May 2 22:26:30 2008
