3f4c

From Proteopedia

(Difference between revisions)

Revision as of 10:27, 9 September 2020

Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound

Structural highlights

3f4c is a 2 chain structure with sequence from Bacillus stearothermophilus 10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	3f4d
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.,Hawwa R, Aikens J, Turner RJ, Santarsiero BD, Mesecar AD Arch Biochem Biophys. 2009 Aug 15;488(2):109-20. Epub 2009 Jul 16. PMID:19615330^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hawwa R, Aikens J, Turner RJ, Santarsiero BD, Mesecar AD. Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus. Arch Biochem Biophys. 2009 Aug 15;488(2):109-20. Epub 2009 Jul 16. PMID:19615330 doi:10.1016/j.abb.2009.06.005

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/3f4c"

@@ Line 1: / Line 1: @@
 ==Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound==
-<StructureSection load='3f4c' size='340' side='right' caption='[[3f4c]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
+<StructureSection load='3f4c' size='340' side='right'caption='[[3f4c]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3f4c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_stearothermophilus_10 Bacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F4C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3f4c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_stearothermophilus_10 Bacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3F4C FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f4d|3f4d]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4c OCA], [http://pdbe.org/3f4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f4c RCSB], [http://www.ebi.ac.uk/pdbsum/3f4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4c ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3f4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4c OCA], [http://pdbe.org/3f4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f4c RCSB], [http://www.ebi.ac.uk/pdbsum/3f4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4c ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Bacillus stearothermophilus 10]]
+[[Category: Large Structures]]
 [[Category: Aikens, J]]
 [[Category: Hawwa, R]]

3f4c

From Proteopedia

Revision as of 10:27, 9 September 2020

Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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