6nqw
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Flagellar protein FcpA from Leptospira biflexa - hexagonal form== | |
| + | <StructureSection load='6nqw' size='340' side='right'caption='[[6nqw]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6nqw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqw OCA], [http://pdbe.org/6nqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nqw RCSB], [http://www.ebi.ac.uk/pdbsum/6nqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The protein FcpA is a unique component of the flagellar filament of spirochete bacteria belonging to the genus Leptospira. Although it plays an essential role in translational motility and pathogenicity, no structures of FcpA homologues are currently available in the PDB. Its three-dimensional structure will unveil the novel motility mechanisms that render pathogenic Leptospira particularly efficient at invading and disseminating within their hosts, causing leptospirosis in humans and animals. FcpA from L. interrogans was purified and crystallized, but despite laborious attempts no useful X ray diffraction data could be obtained. This challenge was solved by expressing a close orthologue from the related saprophytic species L. biflexa. Three different crystal forms were obtained: a primitive and a centred monoclinic form, as well as a hexagonal variant. All forms diffracted X-rays to suitable resolutions for crystallographic analyses, with the hexagonal type typically reaching the highest limits of 2.0 A and better. A variation of the quick-soaking procedure resulted in an iodide derivative that was instrumental for single-wavelength anomalous diffraction methods. | ||
| - | + | Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis.,San Martin F, Mechaly AE, Larrieux N, Wunder EA Jr, Ko AI, Picardeau M, Trajtenberg F, Buschiazzo A Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):123-129. doi:, 10.1107/S2053230X17002096. Epub 2017 Feb 21. PMID:28291747<ref>PMID:28291747</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 6nqw" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Buschiazzo, A]] | [[Category: Buschiazzo, A]] | ||
| - | [[Category: | + | [[Category: Larrieux, N]] |
| + | [[Category: Martin, F San]] | ||
[[Category: Trajtenberg, F]] | [[Category: Trajtenberg, F]] | ||
| + | [[Category: Flagellar filament]] | ||
| + | [[Category: Protein fibril]] | ||
Revision as of 15:30, 29 January 2020
Flagellar protein FcpA from Leptospira biflexa - hexagonal form
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