8h2c
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the pseudaminic acid synthase PseI from Campylobacter jejuni== | |
| + | <StructureSection load='8h2c' size='340' side='right'caption='[[8h2c]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8h2c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H2C FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h2c OCA], [https://pdbe.org/8h2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h2c RCSB], [https://www.ebi.ac.uk/pdbsum/8h2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h2c ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A6C7NV50_CAMJU A0A6C7NV50_CAMJU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Campylobacter jejuni PseI is a pseudaminic acid synthase that condenses the 2,4-diacetamido-2,4,6-trideoxy-l-altrose sugar (6-deoxy AltdiNAc) and phosphoenolpyruvate to generate pseudaminic acid, a sialic acid-like 9-carbon backbone alpha-keto sugar. Pseudaminic acid is conjugated to cell surface proteins and lipids and plays a key role in the mobility and virulence of C. jejuni and other pathogenic bacteria. To provide insights into the catalytic mechanism of PseI, we performed a structural study on PseI. PseI forms a two-domain structure and assembles into a domain-swapped homodimer. The PseI dimer has two cavities, each of which accommodates a metal ion using conserved histidine residues. A comparative analysis of structures and sequences suggests that the cavity of PseI functions as an active site that binds the 6-deoxy AltdiNAc and phosphoenolpyruvate substrates and mediates their condensation. Furthermore, we propose the substrate binding-induced structural rearrangement of PseI and predict 6-deoxy AltdiNAc recognition residues that are specific to PseI. | ||
| - | + | Structural analysis of the pseudaminic acid synthase PseI from Campylobacter jejuni.,Song WS, Park MA, Ki DU, Yoon SI Biochem Biophys Res Commun. 2022 Oct 17;635:252-258. doi:, 10.1016/j.bbrc.2022.10.050. PMID:36283338<ref>PMID:36283338</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8h2c" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Campylobacter jejuni]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ki DU]] | ||
| + | [[Category: Park MA]] | ||
| + | [[Category: Song WS]] | ||
| + | [[Category: Yoon SI]] | ||
Revision as of 07:39, 9 November 2022
Crystal structure of the pseudaminic acid synthase PseI from Campylobacter jejuni
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