1irq

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{{STRUCTURE_1irq| PDB=1irq | SCENE= }}
{{STRUCTURE_1irq| PDB=1irq | SCENE= }}
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'''Crystal structure of omega transcriptional repressor at 1.5A resolution'''
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===Crystal structure of omega transcriptional repressor at 1.5A resolution===
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==Overview==
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The 71 amino acid residue omega protein encoded by the Streptococcus pyogenes non-conjugative plasmid pSM19035 is a transcriptional repressor that regulates expression of genes for copy number control and stable maintenance of plasmids. The crystal structure of omega protein has been determined by multiple isomorphous replacement, including anomalous scattering and refined to an R-factor of 21.1 % (R(free)=23.2 %) at 1.5 A resolution. Two monomers related by a non-crystallographic 2-fold axis form a homodimer that occupies the asymmetric unit. Each polypeptide chain is folded into two alpha-helices and one beta-strand forming an antiparallel beta-ribbon in the homodimer. The N-terminal regions (1-23 and 1-22 in subunits I and II, respectively) are not defined in the electron density due to proteolysis of the N-terminal 20 amino acid residues during crystallisation and partial disorder. The omega protein belongs to the structural superfamily of MetJ/Arc repressors featuring a ribbon-helix-helix DNA-binding motif with the beta-ribbon located in and recognizing the major groove of operator DNA; according to a modelled omega protein-DNA complex, residues Arg31 and Arg31' on the beta-ribbon are in positions to interact with a nucleobase, especially guanine.
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(as it appears on PubMed at http://www.pubmed.gov), where 11733997 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11733997}}
==About this Structure==
==About this Structure==
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[[Category: Ribbon-helix-helix]]
[[Category: Ribbon-helix-helix]]
[[Category: Transcriptional repressor]]
[[Category: Transcriptional repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:53:15 2008''

Revision as of 10:53, 1 July 2008

Image:1irq.png

Template:STRUCTURE 1irq

Crystal structure of omega transcriptional repressor at 1.5A resolution

Template:ABSTRACT PUBMED 11733997

About this Structure

1IRQ is a Single protein structure of sequence from Streptococcus pyogenes. Full crystallographic information is available from OCA.

Reference

Crystal structure of omega transcriptional repressor encoded by Streptococcus pyogenes plasmid pSM19035 at 1.5 A resolution., Murayama K, Orth P, de la Hoz AB, Alonso JC, Saenger W, J Mol Biol. 2001 Dec 7;314(4):789-96. PMID:11733997

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