1v03
From Proteopedia
(New page: 200px<br /> <applet load="1v03" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v03, resolution 2.00Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1V03 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]] with BGC, IPH and CCN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V03 OCA]]. | + | 1V03 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]] with BGC, IPH and CCN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V03 OCA]]. |
==Reference== | ==Reference== | ||
Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15148317 15148317] | Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15148317 15148317] | ||
| + | [[Category: Beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sorghum bicolor]] | [[Category: Sorghum bicolor]] | ||
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[[Category: pest defense]] | [[Category: pest defense]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:31:05 2007'' |
Revision as of 11:26, 30 October 2007
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CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1
Overview
Plant beta-glucosidases play a crucial role in defense against pests. They, cleave, with variable specificity, beta-glucosides to release toxic, aglycone moieties. The Sorghum bicolor beta-glucosidase isoenzyme Dhr1 has, a strict specificity for its natural substrate dhurrin, (p-hydroxy-(S)-mandelonitrile-beta-D-glucoside), whereas its close, homolog, the maize beta-glucosidase isoenzyme Glu1, which shares 72%, sequence identity, hydrolyzes a broad spectrum of substrates in addition, to its natural substrate, 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxaxin-3-one., Structural data from enzyme.substrate complexes of Dhr1 show that the mode, of aglycone binding differs from that previously observed in the, homologous maize enzyme. Specifically, the data suggest that Asn(259), ... [(full description)]
About this Structure
1V03 is a [Single protein] structure of sequence from [Sorghum bicolor] with BGC, IPH and CCN as [ligands]. Active as [Beta-glucosidase], with EC number [3.2.1.21]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:15148317
Page seeded by OCA on Tue Oct 30 13:31:05 2007
Categories: Beta-glucosidase | Single protein | Sorghum bicolor | Bevan, D.R. | Czjzek, M. | Esen, A. | Henrissat, B. | Moriniere, J. | Verdoucq, L. | BGC | CCN | IPH | Dhurrin hydrolysis | Family gh1 | Pest defense
