1jw3
From Proteopedia
(New page: 200px<br /><applet load="1jw3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jw3" /> '''Solution Structure of Methanobacterium Therm...) |
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| - | [[Image:1jw3.gif|left|200px]]<br /><applet load="1jw3" size=" | + | [[Image:1jw3.gif|left|200px]]<br /><applet load="1jw3" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598. Ontario Centre for Structural Proteomics target MTH1598_1_140; Northeast Structural Genomics Target TT6'''<br /> | '''Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598. Ontario Centre for Structural Proteomics target MTH1598_1_140; Northeast Structural Genomics Target TT6'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The influx of genomic sequence information has led to the concept of | + | The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1JW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http:// | + | 1JW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
[[Category: Chang, X.]] | [[Category: Chang, X.]] | ||
[[Category: Connelly, G.]] | [[Category: Connelly, G.]] | ||
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
| - | [[Category: Kennedy, M | + | [[Category: Kennedy, M A.]] |
| - | [[Category: NESG, Northeast | + | [[Category: NESG, Northeast Structural Genomics Consortium.]] |
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
[[Category: beta-alpha-beta sandwich fold]] | [[Category: beta-alpha-beta sandwich fold]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:23 2008'' |
Revision as of 11:27, 21 February 2008
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Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598. Ontario Centre for Structural Proteomics target MTH1598_1_140; Northeast Structural Genomics Target TT6
Overview
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
About this Structure
1JW3 is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485
Page seeded by OCA on Thu Feb 21 13:27:23 2008
Categories: Methanothermobacter thermautotrophicus | Single protein | Arrowsmith, C H. | Chang, X. | Connelly, G. | Edwards, A M. | Kennedy, M A. | NESG, Northeast Structural Genomics Consortium. | Yee, A. | Beta-alpha-beta sandwich fold | Hypothetical protein | Mth1598 | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomics
