2j1k
From Proteopedia
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| - | '''CAV-2 FIBRE HEAD IN COMPLEX WITH CAR D1''' | + | {{Structure |
| + | |PDB= 2j1k |SIZE=350|CAPTION= <scene name='initialview01'>2j1k</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
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| + | '''CAV-2 FIBRE HEAD IN COMPLEX WITH CAR D1''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J1K is a [ | + | 2J1K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canine_adenovirus_2 Canine adenovirus 2] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1K OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor., Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S, J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:[http:// | + | Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor., Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S, J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16923808 16923808] |
[[Category: Canine adenovirus 2]] | [[Category: Canine adenovirus 2]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: virus/receptor complex]] | [[Category: virus/receptor complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:36:10 2008'' |
Revision as of 15:36, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CAV-2 FIBRE HEAD IN COMPLEX WITH CAR D1
Contents |
Overview
Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5A resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer.
Disease
Known diseases associated with this structure: Adrenocortical tumor, somatic OMIM:[188830], Carney complex, type 1 OMIM:[188830], Myxoma, intracardiac OMIM:[188830], Pigmented adrenocortical disease, primary, 1 OMIM:[188830], Spastic paraplegia-7 OMIM:[602783], Thyroid carcinoma, papillary OMIM:[188830]
About this Structure
2J1K is a Protein complex structure of sequences from Canine adenovirus 2 and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor., Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S, J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:16923808
Page seeded by OCA on Thu Mar 20 17:36:10 2008
Categories: Canine adenovirus 2 | Homo sapiens | Protein complex | Billet, O. | Cusack, S. | Kremer, E J. | Lortat-Jacob, H. | Seiradake, E. | Adenovirus | Alternative splicing | Canine | Car | Cav-2 | Cell adhesion | Complex | Coxsackievirus | Domain d1 | Fiber | Fiber head | Fiber protein | Fibre | Fibre head | Glycoprotein | Host-virus interaction | Immunoglobulin domain | Knob | Lipoprotein | Membrane | Palmitate | Phosphorylation | Receptor | Tight junction | Transmembrane | Virus/receptor complex
