4pph
From Proteopedia
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| - | ''' | + | ==Crystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds== |
| + | <StructureSection load='4pph' size='340' side='right' caption='[[4pph]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4pph]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lupinus_angustifolius Lupinus angustifolius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PPH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aup|3aup]], [[3vlb|3vlb]], [[3vla|3vla]], [[3hd8|3hd8]], [[1t6e|1t6e]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pph OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pph RCSB], [http://www.ebi.ac.uk/pdbsum/4pph PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This study describes in vitro digestion of lupin seed globulins by pancreatin, trypsin and chymotrypsin. Lupin seed globulins turned out to be almost totally susceptible to chymotrypsin digestion. When panceratin or trypsin were used for digestion of lupin seed globulins, gamma-conglutin appeared to be resistant to proteolysis. Different fluorescence spectroscopic methods such as fluorescence anisotropy, fluorescence lifetimes and fluorescence quenching measurements were used for detailed characterisation of this phenomenon. A potential reason for gamma-conglutin insensitivity to digestion may be related to the fact that lysine, as well as arginine, are positively charged at cell physiological pH. Simultaneously, flavonoids at this pH are partially ionised, which may lead to the occurrence of ionic interactions between these molecules at pH 7.5. The confirmation of this explanation may be the fact that gamma-conglutin and vitexin form a static complex, which was observed using fluorescence quenching measurements. | ||
| - | + | Characterisation of different digestion susceptibility of lupin seed globulins.,Czubinski J, Dwiecki K, Siger A, Neunert G, Lampart-Szczapa E Food Chem. 2014 Jan 15;143:418-26. doi: 10.1016/j.foodchem.2013.08.015. Epub 2013, Aug 11. PMID:24054261<ref>PMID:24054261</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Lupinus angustifolius]] | ||
[[Category: Barciszewski, J]] | [[Category: Barciszewski, J]] | ||
[[Category: Czubinski, J]] | [[Category: Czubinski, J]] | ||
| + | [[Category: Gilski, M]] | ||
| + | [[Category: Jaskolski, M]] | ||
[[Category: Lampart-Szczapa, E]] | [[Category: Lampart-Szczapa, E]] | ||
| - | [[Category: | + | [[Category: 7s basic globulin]] |
| + | [[Category: Apigenin glycoside]] | ||
| + | [[Category: Glycoside-hydrolase-inhibitor-like protein]] | ||
| + | [[Category: Insulin]] | ||
| + | [[Category: Lupine cotyledon]] | ||
| + | [[Category: N-linked glycosylation]] | ||
| + | [[Category: Non-storage role]] | ||
| + | [[Category: Plant protein]] | ||
Revision as of 11:51, 12 February 2015
Crystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds
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