2qpf
From Proteopedia
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| - | [[Image:2qpf.jpg|left|200px]] | + | [[Image:2qpf.jpg|left|200px]] |
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| - | '''Crystal Structure of Mouse Transthyretin''' | + | {{Structure |
| + | |PDB= 2qpf |SIZE=350|CAPTION= <scene name='initialview01'>2qpf</scene>, resolution 2.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Ttr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Mouse Transthyretin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QPF is a [ | + | 2QPF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QPF OCA]. |
==Reference== | ==Reference== | ||
| - | Human-Murine Transthyretin Heterotetramers Are Kinetically Stable and Non-amyloidogenic: A LESSON IN THE GENERATION OF TRANSGENIC MODELS OF DISEASES INVOLVING OLIGOMERIC PROTEINS., Reixach N, Foss TR, Santelli E, Pascual J, Kelly JW, Buxbaum JN, J Biol Chem. 2008 Jan 25;283(4):2098-107. Epub 2007 Nov 15. PMID:[http:// | + | Human-Murine Transthyretin Heterotetramers Are Kinetically Stable and Non-amyloidogenic: A LESSON IN THE GENERATION OF TRANSGENIC MODELS OF DISEASES INVOLVING OLIGOMERIC PROTEINS., Reixach N, Foss TR, Santelli E, Pascual J, Kelly JW, Buxbaum JN, J Biol Chem. 2008 Jan 25;283(4):2098-107. Epub 2007 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18006495 18006495] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: thyroid hormone]] | [[Category: thyroid hormone]] | ||
[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | [[Category: vitamin | + | [[Category: vitamin some]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:28:08 2008'' |
Revision as of 16:28, 20 March 2008
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| , resolution 2.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | Ttr (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Mouse Transthyretin
Overview
The transthyretin amyloidoses appear to be caused by rate-limiting tetramer dissociation and partial monomer unfolding of the human serum protein transthyretin, resulting in aggregation and extracellular deposition of amorphous aggregates and amyloid fibrils. Mice transgenic for few copies of amyloid-prone human transthyretin variants, including the aggressive L55P mutant, failed to develop deposits. Silencing the murine transthyretin gene in the presence of the L55P human gene resulted in enhanced tissue deposition. To test the hypothesis that the murine protein interacted with human transthyretin, preventing the dissociation and partial unfolding required for amyloidogenesis, we produced recombinant murine transthyretin and human/murine transthyretin heterotetramers and compared their structures and biophysical properties to recombinant human transthyretin. We found no significant differences between the crystal structures of murine and human homotetramers. Murine transthyretin is not amyloidogenic because the native homotetramer is kinetically stable under physiologic conditions and cannot dissociate into partially unfolded monomers, the misfolding and aggregation precursor. Heterotetramers composed of murine and human subunits are also kinetically stable. These observations explain the lack of transthyretin deposition in transgenics carrying a low copy number of human transthyretin genes. The incorporation of mouse subunits into tetramers otherwise composed of human amyloid-prone transthyretin subunits imposes kinetic stability, preventing dissociation and subsequent amyloidogenesis.
About this Structure
2QPF is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Human-Murine Transthyretin Heterotetramers Are Kinetically Stable and Non-amyloidogenic: A LESSON IN THE GENERATION OF TRANSGENIC MODELS OF DISEASES INVOLVING OLIGOMERIC PROTEINS., Reixach N, Foss TR, Santelli E, Pascual J, Kelly JW, Buxbaum JN, J Biol Chem. 2008 Jan 25;283(4):2098-107. Epub 2007 Nov 15. PMID:18006495
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