5u3h
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of apo PCP1 from yersiniabactin synthetase== | |
| + | <StructureSection load='5u3h' size='340' side='right' caption='[[5u3h]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5u3h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U3H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U3H FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u3h OCA], [http://pdbe.org/5u3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u3h RCSB], [http://www.ebi.ac.uk/pdbsum/5u3h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u3h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nonribosomal peptide synthetases (NRPSs) employ multiple domains separated by linker regions to incorporate substrates into natural products. During synthesis, substrates are covalently tethered to carrier proteins that translocate between catalytic partner domains. The molecular parameters that govern translocation and associated linker remodeling remain unknown. Here, we used NMR to characterize the structure, dynamics, and invisible states of a peptidyl carrier protein flanked by its linkers. We showed that the N-terminal linker stabilizes and interacts with the protein core while modulating dynamics at specific sites involved in post-translational modifications and/or domain interactions. The results detail the molecular communication between peptidyl carrier proteins and their linkers and could guide efforts in engineering NRPSs to obtain new pharmaceuticals. | ||
| - | + | Molecular Cross-Talk between Nonribosomal Peptide Synthetase Carrier Proteins and Unstructured Linker Regions.,Harden BJ, Frueh DP Chembiochem. 2017 Jan 24. doi: 10.1002/cbic.201700030. PMID:28120469<ref>PMID:28120469</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5u3h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Frueh, D P]] | ||
| + | [[Category: Harden, B J]] | ||
| + | [[Category: Ligase]] | ||
| + | [[Category: Nrp]] | ||
| + | [[Category: Peptidyl carrier protein]] | ||
Revision as of 09:29, 10 March 2017
Solution structure of apo PCP1 from yersiniabactin synthetase
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