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| - | ==Crystal strukture of Rv0183, a Monoglyceride Lipase from Mycobacterium Tuberculosis== | + | ==Crystal structure of Rv0183, a Monoglyceride Lipase from Mycobacterium Tuberculosis== |
| | <StructureSection load='6eic' size='340' side='right'caption='[[6eic]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6eic' size='340' side='right'caption='[[6eic]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6eic]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EIC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EIC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6eic]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EIC FirstGlance]. <br> |
| | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eic OCA], [http://pdbe.org/6eic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eic RCSB], [http://www.ebi.ac.uk/pdbsum/6eic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eic ProSAT]</span></td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv0183 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eic OCA], [https://pdbe.org/6eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eic RCSB], [https://www.ebi.ac.uk/pdbsum/6eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eic ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MGLL_MYCTU MGLL_MYCTU]] Involved in the hydrolysis of exogenous host lipids during chronic infection. Catalyzes the hydrolysis of both monoacylglycerols (MAG) and diacylglycerols (DAG). It hydrolyzes 2-MAG, 1-3-MAG and MAG with short, medium and long chain fatty acids such as 1-monobutyroyl-rac-glycerol (MC4), 1-mono-octanoyl-rac-glycerol (MC8), 1-monodecanoyl-rac-glycerol (MC10), 1-monolauroyl-rac-glycerol (MC12), 1-monomyristoyl-rac-glycerol (MC14) and 1-mono-oleyl-rac-glycerol (MC18:1) (PubMed:17784850). Also able to hydrolyze DAG with short (DiC6) and medium (DiC10) fatty acid chains, but not whith a longest chain fatty acids (PubMed:17784850). Can also hydrolyze vinyl laurate (VC12), vinyl butyrate (VC4) and vinyl propionate (VC3) (PubMed:17784850).<ref>PMID:17784850</ref> <ref>PMID:22405030</ref> | + | [[https://www.uniprot.org/uniprot/MGLL_MYCTU MGLL_MYCTU]] Involved in the hydrolysis of exogenous host lipids during chronic infection. Catalyzes the hydrolysis of both monoacylglycerols (MAG) and diacylglycerols (DAG). It hydrolyzes 2-MAG, 1-3-MAG and MAG with short, medium and long chain fatty acids such as 1-monobutyroyl-rac-glycerol (MC4), 1-mono-octanoyl-rac-glycerol (MC8), 1-monodecanoyl-rac-glycerol (MC10), 1-monolauroyl-rac-glycerol (MC12), 1-monomyristoyl-rac-glycerol (MC14) and 1-mono-oleyl-rac-glycerol (MC18:1) (PubMed:17784850). Also able to hydrolyze DAG with short (DiC6) and medium (DiC10) fatty acid chains, but not whith a longest chain fatty acids (PubMed:17784850). Can also hydrolyze vinyl laurate (VC12), vinyl butyrate (VC4) and vinyl propionate (VC3) (PubMed:17784850).<ref>PMID:17784850</ref> <ref>PMID:22405030</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | + | [[Category: Myctu]] |
| | [[Category: Aschauer, P]] | | [[Category: Aschauer, P]] |
| | [[Category: Oberer, M]] | | [[Category: Oberer, M]] |
| Structural highlights
Function
[MGLL_MYCTU] Involved in the hydrolysis of exogenous host lipids during chronic infection. Catalyzes the hydrolysis of both monoacylglycerols (MAG) and diacylglycerols (DAG). It hydrolyzes 2-MAG, 1-3-MAG and MAG with short, medium and long chain fatty acids such as 1-monobutyroyl-rac-glycerol (MC4), 1-mono-octanoyl-rac-glycerol (MC8), 1-monodecanoyl-rac-glycerol (MC10), 1-monolauroyl-rac-glycerol (MC12), 1-monomyristoyl-rac-glycerol (MC14) and 1-mono-oleyl-rac-glycerol (MC18:1) (PubMed:17784850). Also able to hydrolyze DAG with short (DiC6) and medium (DiC10) fatty acid chains, but not whith a longest chain fatty acids (PubMed:17784850). Can also hydrolyze vinyl laurate (VC12), vinyl butyrate (VC4) and vinyl propionate (VC3) (PubMed:17784850).[1] [2]
Publication Abstract from PubMed
Monoacylglycerol lipases (MGLs) are enzymes that hydrolyze monoacylglycerol into a free fatty acid and glycerol. Fatty acids can be used for triacylglycerol synthesis, as energy source, as building blocks for energy storage, and as precursor for membrane phospholipids. In Mycobacterium tuberculosis, fatty acids also serve as precursor for polyketide lipids like mycolic acids, major components of the cellular envelope associated to resistance for drug. We present the crystal structure of the MGL Rv0183 from Mycobacterium tuberculosis (mtbMGL) in open conformation. The structure reveals remarkable similarities with MGL from humans (hMGL) in both, the cap region and the alpha/beta core. Nevertheless, mtbMGL could not be inhibited with JZL-184, a known inhibitor of hMGL. Docking studies provide an explanation why the activity of mtbMGL was not affected by the inhibitor. Our findings suggest that specific inhibition of mtbMGL from Mycobacterium tuberculosis, one of the oldest recognized pathogens, is possible without influencing hMGL.
The crystal structure of monoacylglycerol lipase from M. tuberculosis reveals the basis for specific inhibition.,Aschauer P, Zimmermann R, Breinbauer R, Pavkov-Keller T, Oberer M Sci Rep. 2018 Jun 12;8(1):8948. doi: 10.1038/s41598-018-27051-7. PMID:29895832[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cotes K, Dhouib R, Douchet I, Chahinian H, de Caro A, Carriere F, Canaan S. Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids. Biochem J. 2007 Dec 15;408(3):417-27. doi: 10.1042/BJ20070745. PMID:17784850 doi:http://dx.doi.org/10.1042/BJ20070745
- ↑ Saravanan P, Dubey VK, Patra S. Potential selective inhibitors against Rv0183 of Mycobacterium tuberculosis targeting host lipid metabolism. Chem Biol Drug Des. 2012 Jun;79(6):1056-62. doi:, 10.1111/j.1747-0285.2012.01373.x. Epub 2012 Apr 17. PMID:22405030 doi:http://dx.doi.org/10.1111/j.1747-0285.2012.01373.x
- ↑ Aschauer P, Zimmermann R, Breinbauer R, Pavkov-Keller T, Oberer M. The crystal structure of monoacylglycerol lipase from M. tuberculosis reveals the basis for specific inhibition. Sci Rep. 2018 Jun 12;8(1):8948. doi: 10.1038/s41598-018-27051-7. PMID:29895832 doi:http://dx.doi.org/10.1038/s41598-018-27051-7
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