8f7y
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Coxsackievirus A10 frozen at -183 degree embedded in crystalline ice== | |
| + | <StructureSection load='8f7y' size='340' side='right'caption='[[8f7y]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8f7y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_A10 Coxsackievirus A10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F7Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f7y OCA], [https://pdbe.org/8f7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f7y RCSB], [https://www.ebi.ac.uk/pdbsum/8f7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f7y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A7L7QVG9_9ENTO A0A7L7QVG9_9ENTO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. Whether and how crystalline ice affects single-particle cryo-EM is still unclear. Here, single-particle cryo-EM was used to analyze three-dimensional structures of various proteins and viruses embedded in crystalline ice formed at various cooling rates. Low cooling rates led to shrinkage deformation and density distortions on samples having loose structures. Higher cooling rates reduced deformations. Deformation-free proteins in crystalline ice were obtained by modifying the freezing conditions, and reconstructions from these samples revealed a marked improvement over vitreous ice. This procedure also increased the efficiency of cryo-EM structure determinations and was essential for high-resolution reconstructions. | ||
| - | + | Addressing compressive deformation of proteins embedded in crystalline ice.,Shi H, Wu C, Zhang X Structure. 2022 Dec 14:S0969-2126(22)00487-7. doi: 10.1016/j.str.2022.12.001. PMID:36586403<ref>PMID:36586403</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8f7y" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Coxsackievirus A10]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Shi H]] | ||
| + | [[Category: Wu C]] | ||
| + | [[Category: Zhang X]] | ||
Revision as of 07:43, 11 January 2023
Structure of Coxsackievirus A10 frozen at -183 degree embedded in crystalline ice
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