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| | ==Major subunit ComGC from S. pneumoniae Com pseudopili== | | ==Major subunit ComGC from S. pneumoniae Com pseudopili== |
| - | <StructureSection load='6y1h' size='340' side='right'caption='[[6y1h]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='6y1h' size='340' side='right'caption='[[6y1h]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6y1h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strr6 Strr6]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y1H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y1H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6y1h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y1H FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cglC, spr1862 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y1h OCA], [https://pdbe.org/6y1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y1h RCSB], [https://www.ebi.ac.uk/pdbsum/6y1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y1h ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y1h OCA], [http://pdbe.org/6y1h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y1h RCSB], [http://www.ebi.ac.uk/pdbsum/6y1h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y1h ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8DN88_STRR6 Q8DN88_STRR6] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strr6]] | + | [[Category: Streptococcus pneumoniae R6]] |
| - | [[Category: Berry, J L]] | + | [[Category: Berry JL]] |
| - | [[Category: Matthews, S J]] | + | [[Category: Matthews SJ]] |
| - | [[Category: Pelicic, V]] | + | [[Category: Pelicic V]] |
| - | [[Category: Sheppard, D]] | + | [[Category: Sheppard D]] |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Type iv filaments type iv pilin competence pseudopili mainly alpha orthogonal bundle]]
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| Structural highlights
Function
Q8DN88_STRR6
Publication Abstract from PubMed
Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major monoderm class of Firmicutes. This also revealed that ComGC displays extensive sequence conservation, defining a monophyletic group among type IV pilins. We further report ComGC solution structures from two naturally competent human pathogens, Streptococcus sanguinis (ComGCSS) and Streptococcus pneumoniae (ComGCSP), revealing that this pilin displays extensive structural conservation. Strikingly, ComGCSS and ComGCSP exhibit a novel type IV pilin fold that is purely helical. Results from homology modelling analyses suggest that ComGC unusual structure is compatible with helical filament assembly. Because ComGC displays such a widespread distribution, these results have implications for hundreds of monoderm species.
The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold.,Sheppard D, Berry JL, Denise R, Rocha EPC, Matthews S, Pelicic V J Biol Chem. 2020 Apr 9. pii: RA120.013316. doi: 10.1074/jbc.RA120.013316. PMID:32273343[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sheppard D, Berry JL, Denise R, Rocha EPC, Matthews S, Pelicic V. The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold. J Biol Chem. 2020 Apr 9. pii: RA120.013316. doi: 10.1074/jbc.RA120.013316. PMID:32273343 doi:http://dx.doi.org/10.1074/jbc.RA120.013316
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