2a8f

From Proteopedia

(Difference between revisions)

Revision as of 07:20, 23 August 2023

beta-cinnamomin after sterol removal

Structural highlights

2a8f is a 2 chain structure with sequence from Phytophthora cinnamomi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.35Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ELIB_PHYCI Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. Elicitins are able to load, carry, and transfer sterols between membranes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.

Crystal structures of the free and sterol-bound forms of beta-cinnamomin.,Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA. Crystal structures of the free and sterol-bound forms of beta-cinnamomin. Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127 doi:http://dx.doi.org/10.1016/j.bbapap.2005.09.008

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/2a8f"

@@ Line 3: / Line 3: @@
 <StructureSection load='2a8f' size='340' side='right'caption='[[2a8f]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2a8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cinnamon_fungus Cinnamon fungus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8F FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2a8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8F FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CIN1B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4785 Cinnamon fungus])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8f OCA], [https://pdbe.org/2a8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8f RCSB], [https://www.ebi.ac.uk/pdbsum/2a8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8f ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/ELIB_PHYCI ELIB_PHYCI]] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. Elicitins are able to load, carry, and transfer sterols between membranes.
+[https://www.uniprot.org/uniprot/ELIB_PHYCI ELIB_PHYCI] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. Elicitins are able to load, carry, and transfer sterols between membranes.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Cinnamon fungus]]
 [[Category: Large Structures]]
-[[Category: Archer, M]]
+[[Category: Phytophthora cinnamomi]]
-[[Category: Baptista, R P]]
+[[Category: Archer M]]
-[[Category: Carrondo, M A]]
+[[Category: Baptista RP]]
-[[Category: Cravador, A]]
+[[Category: Carrondo MA]]
-[[Category: Enguita, F J]]
+[[Category: Cravador A]]
-[[Category: Martel, P]]
+[[Category: Enguita FJ]]
-[[Category: Melo, E P]]
+[[Category: Martel P]]
-[[Category: Miranda, S]]
+[[Category: Melo EP]]
-[[Category: Rodrigues, M L]]
+[[Category: Miranda S]]
-[[Category: Sousa, N]]
+[[Category: Rodrigues ML]]
-[[Category: Thomaz, M]]
+[[Category: Sousa N]]
-[[Category: Elicitin]]
+[[Category: Thomaz M]]
-[[Category: Phytopathogen]]
-[[Category: Phytophthora]]
-[[Category: Sterol carrier protein]]
-[[Category: Toxin]]

2a8f

From Proteopedia

Revision as of 07:20, 23 August 2023

beta-cinnamomin after sterol removal

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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