2fe9

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of the Vts1 SAM domain in the presence of RNA

Structural highlights

2fe9 is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VTS1_YEAST RNA-binding protein involved in post-transcriptional regulation through transcript degradation of SRE (SMG-recognition elements) bearing mRNAs. May be involved in vacuolar protein transport.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The yeast Vts1 SAM (sterile alpha motif) domain is a member of a new class of SAM domains that specifically bind RNA. To elucidate the structural basis for RNA binding, the solution structure of the Vts1 SAM domain, in the presence of a specific target RNA, has been solved by multidimensional heteronuclear NMR spectroscopy. The Vts1 SAM domain retains the "core" five-helix-bundle architecture of traditional SAM domains, but has additional short helices at N and C termini, comprising a small substructure that caps the core helices. The RNA-binding surface of Vts1, determined by chemical shift perturbation, maps near the ends of three of the core helices, in agreement with mutational data and the electrostatic properties of the molecule. These results provide a structural basis for the versatility of the SAM domain in protein and RNA-recognition.

Solution structure of the Vts1 SAM domain in the presence of RNA.,Edwards TA, Butterwick JA, Zeng L, Gupta YK, Wang X, Wharton RP, Palmer AG 3rd, Aggarwal AK J Mol Biol. 2006 Mar 10;356(5):1065-72. Epub 2005 Dec 20. PMID:16405996^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dilcher M, Kohler B, von Mollard GF. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. J Biol Chem. 2001 Sep 14;276(37):34537-44. Epub 2001 Jul 9. PMID:11445562 doi:http://dx.doi.org/10.1074/jbc.M101551200
↑ Aviv T, Lin Z, Lau S, Rendl LM, Sicheri F, Smibert CA. The RNA-binding SAM domain of Smaug defines a new family of post-transcriptional regulators. Nat Struct Biol. 2003 Aug;10(8):614-21. PMID:12858164 doi:10.1038/nsb956
↑ Edwards TA, Butterwick JA, Zeng L, Gupta YK, Wang X, Wharton RP, Palmer AG 3rd, Aggarwal AK. Solution structure of the Vts1 SAM domain in the presence of RNA. J Mol Biol. 2006 Mar 10;356(5):1065-72. Epub 2005 Dec 20. PMID:16405996 doi:10.1016/j.jmb.2005.12.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/2fe9"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2fe9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FE9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fe9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fe9 OCA], [https://pdbe.org/2fe9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fe9 RCSB], [https://www.ebi.ac.uk/pdbsum/2fe9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fe9 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fe9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fe9 OCA], [https://pdbe.org/2fe9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fe9 RCSB], [https://www.ebi.ac.uk/pdbsum/2fe9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fe9 ProSAT]</span></td></tr>
 </table>
 == Function ==

2fe9

From Proteopedia

Current revision

Solution structure of the Vts1 SAM domain in the presence of RNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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