2a8f
From Proteopedia
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'''beta-cinnamomin after sterol removal''' | '''beta-cinnamomin after sterol removal''' | ||
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[[Category: Sousa, N.]] | [[Category: Sousa, N.]] | ||
[[Category: Thomaz, M.]] | [[Category: Thomaz, M.]] | ||
| - | [[Category: | + | [[Category: Elicitin]] |
| - | [[Category: | + | [[Category: Phytopathogen]] |
| - | [[Category: | + | [[Category: Phytophthora]] |
| - | [[Category: | + | [[Category: Sterol carrier protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:44:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:44, 3 May 2008
beta-cinnamomin after sterol removal
Overview
The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
About this Structure
2A8F is a Single protein structure of sequence from Phytophthora cinnamomi. Full crystallographic information is available from OCA.
Reference
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127 Page seeded by OCA on Sat May 3 18:44:29 2008
