1fsd

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[[Image:1fsd.gif|left|200px]]
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==Overview==
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{{ABSTRACT_9311930}}
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The first fully automated design and experimental validation of a novel sequence for an entire protein is described. A computational design algorithm based on physical chemical potential functions and stereochemical constraints was used to screen a combinatorial library of 1.9 x 10(27) possible amino acid sequences for compatibility with the design target, a betabetaalpha protein motif based on the polypeptide backbone structure of a zinc finger domain. A BLAST search shows that the designed sequence, full sequence design 1 (FSD-1), has very low identity to any known protein sequence. The solution structure of FSD-1 was solved by nuclear magnetic resonance spectroscopy and indicates that FSD-1 forms a compact well-ordered structure, which is in excellent agreement with the design target structure. This result demonstrates that computational methods can perform the immense combinatorial search required for protein design, and it suggests that an unbiased and quantitative algorithm can be used in various structural contexts.
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==About this Structure==
==About this Structure==
The following page contains interesting information on the relation of 1FSD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSD OCA].
The following page contains interesting information on the relation of 1FSD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSD OCA].
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==Reference==
 
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De novo protein design: fully automated sequence selection., Dahiyat BI, Mayo SL, Science. 1997 Oct 3;278(5335):82-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9311930 9311930]
 
[[Category: Designer Proteins]]
[[Category: Designer Proteins]]
[[Category: Dahiyat, B I.]]
[[Category: Dahiyat, B I.]]
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[[Category: Computational design]]
[[Category: Computational design]]
[[Category: Novel sequence]]
[[Category: Novel sequence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:42:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:23:30 2008''

Revision as of 14:23, 26 June 2008

Image:1fsd.png

Template:STRUCTURE 1fsd

FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, 41 STRUCTURES


Template:ABSTRACT 9311930

About this Structure

The following page contains interesting information on the relation of 1FSD with [Designer Proteins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:23:30 2008

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