2a8f

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[[Image:2a8f.gif|left|200px]]
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{{STRUCTURE_2a8f| PDB=2a8f | SCENE= }}
{{STRUCTURE_2a8f| PDB=2a8f | SCENE= }}
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'''beta-cinnamomin after sterol removal'''
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===beta-cinnamomin after sterol removal===
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==Overview==
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The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
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The line below this paragraph, {{ABSTRACT_PUBMED_16249127}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16249127 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16249127}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16249127 16249127]
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16249127 16249127]
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Structure of beta-cinnamomin, a protein toxic to some plant species., Rodrigues ML, Archer M, Martel P, Jacquet A, Cravador A, Carrondo MA, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1314-21. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136143 12136143]
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Identification of an elicitin gene cluster in Phytophthora cinnamomi., Duclos J, Fauconnier A, Coelho AC, Bollen A, Cravador A, Godfroid E, DNA Seq. 1998;9(4):231-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10520754 10520754]
[[Category: Phytophthora cinnamomi]]
[[Category: Phytophthora cinnamomi]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Phytophthora]]
[[Category: Phytophthora]]
[[Category: Sterol carrier protein]]
[[Category: Sterol carrier protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:44:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:53:04 2008''

Revision as of 11:53, 29 July 2008

Image:2a8f.png

Template:STRUCTURE 2a8f

beta-cinnamomin after sterol removal

Template:ABSTRACT PUBMED 16249127

About this Structure

2A8F is a Single protein structure of sequence from Phytophthora cinnamomi. Full crystallographic information is available from OCA.

Reference

Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127

Structure of beta-cinnamomin, a protein toxic to some plant species., Rodrigues ML, Archer M, Martel P, Jacquet A, Cravador A, Carrondo MA, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1314-21. Epub 2002, Jul 20. PMID:12136143

Identification of an elicitin gene cluster in Phytophthora cinnamomi., Duclos J, Fauconnier A, Coelho AC, Bollen A, Cravador A, Godfroid E, DNA Seq. 1998;9(4):231-7. PMID:10520754

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