1a0r

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spinqualitylabelsall models 1a0r, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

HETEROTRIMERIC COMPLEX OF PHOSDUCIN/TRANSDUCIN BETA-GAMMA

Overview

BACKGROUND: Phosducin binds tightly to the beta gamma subunits (Gt beta, gamma) of the heterotrimeric G protein transducin, preventing Gt beta, gamma reassociation with Gt alpha-GDP and thereby inhibiting the G-protein, cycle. Phosducin-like proteins appear to be widely distributed and may, play important roles in regulating many heterotrimeric G-protein signaling, pathways. RESULTS: The 2.8 A crystal structure of a complex of bovine, retinal phosducin with Gt beta gamma shows how the two domains of, phosducin cover one side and the top of the seven-bladed beta propeller of, Gt beta gamma. The binding of phosducin induces a distinct structural, change in the beta propeller of Gt beta gamma, such that a small cavity, opens up between blades 6 and 7. Electron density in this cavity has been, assigned to the farnesyl moiety of the gamma subunit. CONCLUSIONS: beta, gamma subunits of heterotrimeric G proteins can exist in two distinct, conformations. In the R (relaxed) state, corresponding to the structure of, the free beta gamma or the structure of beta gamma in the alpha beta gamma, heterotrimer, the hydrophobic farnesyl moiety of the gamma subunit is, exposed, thereby mediating membrane association. In the T (tense) state, as observed in the phosducin-Gt beta gamma structure, the farnesyl moiety, of the gamma subunit is effectively buried in the cavity formed between, blades 6 and 7 of the beta subunit. Binding of phosducin to Gt beta gamma, induces the formation of this cavity, resulting in a switch from the R to, the T conformation. This sequesters beta gamma from the membrane to the, cytosol and turns off the signal-transduction cascade. Regulation of this, membrane association/dissociation switch of Gt beta gamma by phosducin may, be a general mechanism for attenuation of G protein coupled signal, transduction cascades.

About this Structure

1A0R is a Protein complex structure of sequences from Bos taurus with ACE and FAR as ligands. Full crystallographic information is available from OCA.

Reference

Phosducin induces a structural change in transducin beta gamma., Loew A, Ho YK, Blundell T, Bax B, Structure. 1998 Aug 15;6(8):1007-19. PMID:9739091

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