2ztj

Structural highlights

Function

HOSA_THET2 Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate (PubMed:19996101, PubMed:12095615). Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway (PubMed:9868782). To a lesser extent, can also use oxaloacetate in place of 2-oxoglutarate, leading to citrate. Does not display 2-isopropylmalate synthase activity since it cannot use 2-oxoisovalerate (PubMed:12095615).^{[1] [2] [3]}

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Homocitrate synthase

References

1. ↑ Wulandari AP, Miyazaki J, Kobashi N, Nishiyama M, Hoshino T, Yamane H. Characterization of bacterial homocitrate synthase involved in lysine biosynthesis. FEBS Lett. 2002 Jul 3;522(1-3):35-40. PMID:12095615 doi:10.1016/s0014-5793(02)02877-6
2. ↑ Okada T, Tomita T, Wulandari AP, Kuzuyama T, Nishiyama M. Mechanism of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus. J Biol Chem. 2010 Feb 5;285(6):4195-205. Epub 2009 Dec 7. PMID:19996101 doi:10.1074/jbc.M109.086330
3. ↑ Kosuge T, Hoshino T. Lysine is synthesized through the alpha-aminoadipate pathway in Thermus thermophilus. FEMS Microbiol Lett. 1998 Dec 15;169(2):361-7. PMID:9868782 doi:10.1111/j.1574-6968.1998.tb13341.x