1bp7

From Proteopedia

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Structural highlights

1bp7 is a 8 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Ligands:	CA
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNE1_CHLRE Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.,Jurica MS, Monnat RJ Jr, Stoddard BL Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

19 reviews cite this structure

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

Chevalier et al. (2001)

18 more

75 other articles

No citations found

References

↑ Jurica MS, Monnat RJ Jr, Stoddard BL. DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI. Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

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PDB ID 1bp7

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Categories: Chlamydomonas reinhardtii | Large Structures | Jurica MS | Monnat Junior RJ | Stoddard BL

1bp7

From Proteopedia

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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