1i0v

From Proteopedia

Ribonuclease T1 in complex with 2'GMP (form I crystal)

Structural highlights

1i0v is a 1 chain structure with sequence from Aspergillus oryzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.234Å
Ligands:	2GP, CA
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNT1_ASPOR

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In the crystalline state, ribonuclease T1 binds calcium ions at different lattice-dependent positions. In solution, its conformational stability is also remarkably increased in the presence of divalent metal ions. Combining urea unfolding studies and X-ray crystallography, we compared the presence of several metal ions at specific sites in the protein to their contribution to the overall stabilizing effect in solution. We constructed thermodynamic cycles involving particular metal ions and specific carboxylate functions. The resulting coupling energies indicate that some (but not all) metal ions found at lattice contacts in crystal structures may indeed significantly contribute to stability enhancement in the presence of metal ions in solution.

The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.,Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:11453993^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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Flexibility of metal binding sites in proteins on a database scale.

Babor et al. (2005)

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3 other articles

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References

↑ Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R. The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution. Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:11453993

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

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PDB ID 1i0v

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1i0v

From Proteopedia

Ribonuclease T1 in complex with 2'GMP (form I crystal)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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