1l5j

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CRYSTAL STRUCTURE OF E. COLI ACONITASE B.

Structural highlights

1l5j is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:F3S, TRA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACNB_ECOLI Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnB functions as a RNA-binding regulatory protein. During oxidative stress inactive AcnB apo-enzyme without iron sulfur clusters binds the acnB mRNA 3' UTRs (untranslated regions), stabilizes acnB mRNA and increases AcnB synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.[1] [2] [3] [4] [5] [6] [7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Jordan PA, Tang Y, Bradbury AJ, Thomson AJ, Guest JR. Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB). Biochem J. 1999 Dec 15;344 Pt 3(Pt 3):739-46 PMID:10585860
  2. ↑ Tang Y, Guest JR. Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases. Microbiology (Reading). 1999 Nov;145 ( Pt 11):3069-3079. PMID:10589714 doi:10.1099/00221287-145-11-3069
  3. ↑ Tang Y, Quail MA, Artymiuk PJ, Guest JR, Green J. Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression. Microbiology (Reading). 2002 Apr;148(Pt 4):1027-1037. PMID:11932448 doi:10.1099/00221287-148-4-1027
  4. ↑ Brock M, Maerker C, Schütz A, Völker U, Buckel W. Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase. Eur J Biochem. 2002 Dec;269(24):6184-94. PMID:12473114 doi:10.1046/j.1432-1033.2002.03336.x
  5. ↑ Tang Y, Guest JR, Artymiuk PJ, Green J. Switching aconitase B between catalytic and regulatory modes involves iron-dependent dimer formation. Mol Microbiol. 2005 Jun;56(5):1149-58. PMID:15882410 doi:10.1111/j.1365-2958.2005.04610.x
  6. ↑ Gruer MJ, Guest JR. Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology (Reading). 1994 Oct;140 ( Pt 10):2531-41. PMID:8000525 doi:10.1099/00221287-140-10-2531
  7. ↑ Bradbury AJ, Gruer MJ, Rudd KE, Guest JR. The second aconitase (AcnB) of Escherichia coli. Microbiology (Reading). 1996 Feb;142 ( Pt 2):389-400. PMID:8932712 doi:10.1099/13500872-142-2-389

Contents


PDB ID 1l5j

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