Structural highlights
Function
3L21_LATSE Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=1.6 nM) and neuronal (chimeric alpha-7/CHRNA7, Kd=3 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). Also shows a very weak inhibition on GABA(A) receptors (PubMed:26221036). The toxin (10 uM) inhibits 83% of current in channels composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) subunits, 39% of current in channels composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits, and 33% of current in channels composed of alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:26221036).[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Kudryavtsev DS, Shelukhina IV, Son LV, Ojomoko LO, Kryukova EV, Lyukmanova EN, Zhmak MN, Dolgikh DA, Ivanov IA, Kasheverov IE, Starkov VG, Ramerstorfer J, Sieghart W, Tsetlin VI, Utkin YN. Neurotoxins from snake venoms and α-conotoxin ImI inhibit functionally active ionotropic γ-aminobutyric acid (GABA) receptors. J Biol Chem. 2015 Sep 11;290(37):22747-58. PMID:26221036 doi:10.1074/jbc.M115.648824
- ↑ Servent D, Winckler-Dietrich V, Hu HY, Kessler P, Drevet P, Bertrand D, Menez A. Only snake curaremimetic toxins with a fifth disulfide bond have high affinity for the neuronal alpha7 nicotinic receptor. J Biol Chem. 1997 Sep 26;272(39):24279-86. PMID:9305882