1n3j

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Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

Structural highlights

1n3j is a 2 chain structure with sequence from Paramecium bursaria Chlorella virus 1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O41094_PBCV1

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.

A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3.,Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
23 reviews cite this structure
The SET-domain protein superfamily: protein lysine methyltransferases.
Dillon et al. (2005)
22 more
35 other articles
No citations found

See Also

References

  1. ↑ Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM. A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3. Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185 doi:10.1038/nsb898

Contents


PDB ID 1n3j

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