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1ntx

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SECONDARY STRUCTURE DETERMINATION FOR ALPHA-NEUROTOXIN FROM DENDROASPIS POLYLEPIS POLYLEPIS BASED ON SEQUENCE SPECIFIC PROTON NUCLEAR MAGNETIC RESONANCE ASSIGNMENTS

Structural highlights

1ntx is a 1 chain structure with sequence from Dendroaspis polylepis polylepis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

3S11_DENPO Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission.[UniProtKB:P60775]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sequence-specific assignments are presented for the polypeptide backbone protons and a majority of the amino-acid-side-chain protons of alpha-neurotoxin from Dendroaspis polylepis polylepis, and individual amide proton-exchange rates with the solvent are reported. The secondary structure and the hydrogen-bonding patterns in the regular secondary structure elements are deduced from nuclear Overhauser effects and the sequence locations of the slowly exchanging amide protons. The molecule includes a three-stranded antiparallel beta-sheet, and there are indications that two additional short chain segments are arranged in an antiparallel beta-sheet. These structural elements are similar, but not identical, to either the secondary structure reported for erabutoxin b in single crystals, or the solution structure of cytotoxin CTXIIb from Naja mossambica mossambica.

Secondary structure determination for alpha-neurotoxin from Dendroaspis polylepis polylepis based on sequence-specific 1H-nuclear-magnetic-resonance assignments.,Labhardt AM, Hunziker-Kwik EH, Wuthrich K Eur J Biochem. 1988 Nov 1;177(2):295-305. PMID:2847926^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Labhardt AM, Hunziker-Kwik EH, Wuthrich K. Secondary structure determination for alpha-neurotoxin from Dendroaspis polylepis polylepis based on sequence-specific 1H-nuclear-magnetic-resonance assignments. Eur J Biochem. 1988 Nov 1;177(2):295-305. PMID:2847926