1rfd

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ANTI-COCAINE ANTIBODY M82G2

Structural highlights

1rfd is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.09Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Antibodies against cocaine and other drugs of abuse are the basis for diagnostic tests for the presence of those drugs in human serum. The 1.7A resolution crystal structure of the anti-cocaine monoclonal antibody M82G2 in complex with cocaine is presented. This structure determination was undertaken to establish the stereochemical features in the antibody binding site that confer specificity for cocaine, and as part of an ongoing project to understand the rules that govern molecular recognition. The cocaine-binding site can be characterized topologically as a narrow groove on the protein surface. The antibody utilizes water-mediated hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to provide specificity. Comparison with the previously published structure of the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can be achieved in diverse ways, both in terms of a binding site structure/topology and protein-ligand interactions.

Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2.,Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D J Mol Biol. 2005 Jun 10;349(3):570-82. Epub 2005 Apr 21. PMID:15885702[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
0 reviews cite this structure
Modeling the catalysis of anti-cocaine catalytic antibody: competing reaction pathways and free energy barriers.
Pan et al. (2008)
-1 more
20 other articles
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See Also

References

  1. ↑ Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D. Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2. J Mol Biol. 2005 Jun 10;349(3):570-82. Epub 2005 Apr 21. PMID:15885702 doi:http://dx.doi.org/10.1016/j.jmb.2005.03.080

Contents


PDB ID 1rfd

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