Structural highlights
Function
PLYL_DICD3 Presents an endo-cleaving activity on polygalacturonate or partially methylated pectin. Is effective in the maceration of plant tissue, and has an important role in soft-rot disease. Is 280-fold less active against polygalacturonate than the major pectate lyase PelB. When assayed on polygalacturonate, PelL releases oligogalacturonates of different sizes; upon prolonged incubation, PelL degrades the primary products to unsaturated tetramer and pentamer in addition to unsaturated dimer and trimer. When assayed on oligogalacturonates (degrees of polymerization of 2 to 8), it preferentially forms unsaturated tetramer, and displays the highest activity on the octamer.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Roy C, Kester H, Visser J, Shevchik V, Hugouvieux-Cotte-Pattat N, Robert-Baudouy J, Benen J. Modes of action of five different endopectate lyases from Erwinia chrysanthemi 3937. J Bacteriol. 1999 Jun;181(12):3705-9. PMID:10368144 doi:10.1128/JB.181.12.3705-3709.1999
- ↑ Lojkowska E, Masclaux C, Boccara M, Robert-Baudouy J, Hugouvieux-Cotte-Pattat N. Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937. Mol Microbiol. 1995 Jun;16(6):1183-95. PMID:8577252 doi:10.1111/j.1365-2958.1995.tb02341.x