1vbs

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STRUCTURE OF CYCLOPHILIN COMPLEXED WITH (D)ALA CONTAINING TETRAPEPTIDE

Structural highlights

1vbs is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:DAL, NIT
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PPIA_HUMAN PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The stereospecificity of peptidyl prolyl cis/trans isomerases (PPIases) was studied using tetrapeptide substrate analogs in which one amino acid residue was replaced by the cognate D-amino acid in various positions of the peptide chain. Reversed stereocenters around proline markedly increased the rate of the spontaneous trans to cis isomerization of the prolyl bond whereas cis to trans isomerizations were less sensitive. PPIases like human cyclophilin18, human FKBP12, Escherichia coli parvulin10 and the PPIase domain of E. coli trigger factor exhibited stereoselectivity demanding at the P1 to P2' position of the substrate chain. The discriminating factor for stereoselectivity was the lack of formation of the Michaelis complexes of the diastereomeric substrates. However, D-alanine at the P1 position preserved considerable affinity to the active site, and largely prevented activation of the catalytic machinery for all PPIases investigated.

Mapping the stereospecificity of peptidyl prolyl cis/trans isomerases.,Schiene C, Reimer U, Schutkowski M, Fischer G FEBS Lett. 1998 Aug 7;432(3):202-6. PMID:9720925[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
The involvement of mammalian and plant FK506-binding proteins (FKBPs) in development.
Breiman et al. (2002)
2 more
5 other articles
No citations found

See Also

References

  1. ↑ Schiene C, Reimer U, Schutkowski M, Fischer G. Mapping the stereospecificity of peptidyl prolyl cis/trans isomerases. FEBS Lett. 1998 Aug 7;432(3):202-6. PMID:9720925

Contents


PDB ID 1vbs

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OCA

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