1vfp

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Crystal structure of the SR CA2+-ATPase with bound AMPPCP

Structural highlights

1vfp is a 2 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:ACP, CA, MG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AT2A1_RABIT This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

P-type ATPases are ATP-powered ion pumps that establish ion concentration gradients across cell and organelle membranes. Here, we describe the crystal structure of the Ca2+ pump of skeletal muscle sarcoplasmic reticulum, a representative member of the P-type ATPase superfamily, with an ATP analogue, a Mg2+ and two Ca2+ ions in the respective binding sites. In this state, the ATP analogue reorganizes the three cytoplasmic domains (A, N and P), which are widely separated without nucleotide, by directly bridging the N and P domains. The structure of the P-domain itself is altered by the binding of the ATP analogue and Mg2+. As a result, the A-domain is tilted so that one of the transmembrane helices moves to lock the cytoplasmic gate of the transmembrane Ca2+-binding sites. This appears to be the mechanism for occluding the bound Ca2+ ions, before releasing them into the lumen of the sarcoplasmic reticulum.

Crystal structure of the calcium pump with a bound ATP analogue.,Toyoshima C, Mizutani T Nature. 2004 Jul 29;430(6999):529-35. Epub 2004 Jun 30. PMID:15229613[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
52 reviews cite this structure
P-type ATPases.
Palmgren et al. (2011)
51 more
165 other articles
No citations found

See Also

References

  1. ↑ Toyoshima C, Mizutani T. Crystal structure of the calcium pump with a bound ATP analogue. Nature. 2004 Jul 29;430(6999):529-35. Epub 2004 Jun 30. PMID:15229613 doi:http://dx.doi.org/10.1038/nature02680

Contents


PDB ID 1vfp

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