2bir

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ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)

Structural highlights

2bir is a 1 chain structure with sequence from Aspergillus oryzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:2GP, CA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNT1_ASPOR

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

It has been established that Tyr-42, Tyr-45, and Glu-46 take part in a structural motif that renders guanine specificity to ribonuclease T1. We report on the impact of Tyr-42, Tyr-45, and Glu-46 substitutions on the guanine specificity of RNase T1. The Y42A and E46A mutations profoundly affect substrate binding. No such effect is observed for Y45A RNase T1. From the kinetics of the Y42A/Y45A and Y42A/E46A double mutants, we conclude that these pairs of residues contribute to guanine specificity in a mutually independent way. From our results, it appears that the energetic contribution of aromatic face-to-face stacking interactions may be significant if polycyclic molecules, such as guanine, are involved.

Additivity of protein-guanine interactions in ribonuclease T1.,Loverix S, Doumen J, Steyaert J J Biol Chem. 1997 Apr 11;272(15):9635-9. PMID:9092491[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
2 reviews cite this structure
A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.
Steyaert et al. (1997)
1 more
4 other articles
No citations found

See Also

References

  1. ↑ Loverix S, Doumen J, Steyaert J. Additivity of protein-guanine interactions in ribonuclease T1. J Biol Chem. 1997 Apr 11;272(15):9635-9. PMID:9092491

Contents


PDB ID 2bir

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OCA

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