2gcb

Structural highlights

Function

FPGS_LACCA Involved in the conversion of folates to polyglutamate derivatives, and likely functions in the retention of cellular folate pools. Catalyzes successive MgATP-dependent additions of glutamate to a pteroylmonoglutamate substrate, with a high preference for 5,10-methylenetetrahydrofolate (mTHF). Thus, metabolizes mTHF to the tetraglutamate derivative, but longer glutamate chain length products are not observed. Tetrahydrofolate (H4PteGlu) and 10-formyl-H4PteGlu are poorer folate substrates. In contrast to E.coli FolC, this enzyme does not display dihydrofolate synthase activity.^{[1] [2]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Folylpolyglutamate synthase

References

1. ↑ Sheng Y, Khanam N, Tsaksis Y, Shi XM, Lu QS, Bognar AL. Mutagenesis of folylpolyglutamate synthetase indicates that dihydropteroate and tetrahydrofolate bind to the same site. Biochemistry. 2008 Feb 26;47(8):2388-96. PMID:18232714 doi:10.1021/bi701670y
2. ↑ Bognar AL, Shane B. Purification and properties of Lactobacillus casei folylpoly-gamma-glutamate synthetase. J Biol Chem. 1983 Oct 25;258(20):12574-81 PMID:6138353