2id0

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Escherichia coli RNase II

Structural highlights

2id0 is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:MN
NonStd Res:MSE
Gene:rnb (ECOLI)
Activity:Exoribonuclease II, with EC number 3.1.13.1
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RNB_ECOLI] Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

RNase II is a member of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hydrolytic enzymes that play an important role in mRNA decay. Here, we report the crystal structure of E. coli RNase II, which reveals an architecture reminiscent of the RNA exosome. Three RNA-binding domains come together to form a clamp-like assembly, which can only accommodate single-stranded RNA. This leads into a narrow, basic channel that ends at the putative catalytic center that is completely enclosed within the body of the protein. The putative path for RNA agrees well with biochemical data indicating that a 3' single strand overhang of 7-10 nt is necessary for binding and hydrolysis by RNase II. The presence of the clamp and the narrow channel provides an explanation for the processivity of RNase II and for why its action is limited to single-stranded RNA.

Structural basis for processivity and single-strand specificity of RNase II.,Zuo Y, Vincent HA, Zhang J, Wang Y, Deutscher MP, Malhotra A Mol Cell. 2006 Oct 6;24(1):149-56. Epub 2006 Sep 21. PMID:16996291[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
15 reviews cite this structure
Nucleases: diversity of structure, function and mechanism.
Yang et al. (2011)
14 more
45 other articles
No citations found

See Also

References

  1. ↑ Cheng ZF, Deutscher MP. Purification and characterization of the Escherichia coli exoribonuclease RNase R. Comparison with RNase II. J Biol Chem. 2002 Jun 14;277(24):21624-9. Epub 2002 Apr 10. PMID:11948193 doi:http://dx.doi.org/10.1074/jbc.M202942200
  2. ↑ Zuo Y, Vincent HA, Zhang J, Wang Y, Deutscher MP, Malhotra A. Structural basis for processivity and single-strand specificity of RNase II. Mol Cell. 2006 Oct 6;24(1):149-56. Epub 2006 Sep 21. PMID:16996291 doi:10.1016/j.molcel.2006.09.004

Contents


PDB ID 2id0

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OCA

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