2k0g

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Solution Structure of a Bacterial Cyclic Nucleotide-Activated K+ Channel Binding Domain in Complex with cAMP

Structural highlights

2k0g is a 1 chain structure with sequence from Mesorhizobium loti. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:CMP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNGK1_RHILO Cyclic nucleotide-regulated potassium channel activated by cAMP.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cyclic nucleotide-sensitive ion channels, known as HCN and CNG channels, are crucial in neuronal excitability and signal transduction of sensory cells. HCN and CNG channels are activated by binding of cyclic nucleotides to their intracellular cyclic nucleotide-binding domain (CNBD). However, the mechanism by which the binding of cyclic nucleotides opens these channels is not well understood. Here, we report the solution structure of the isolated CNBD of a cyclic nucleotide-sensitive K(+) channel from Mesorhizobium loti. The protein consists of a wide anti-parallel beta-roll topped by a helical bundle comprising five alpha-helices and a short 3(10)-helix. In contrast to the dimeric arrangement ('dimer-of-dimers') in the crystal structure, the solution structure clearly shows a monomeric fold. The monomeric structure of the CNBD supports the hypothesis that the CNBDs transmit the binding signal to the channel pore independently of each other.

Solution structure of the Mesorhizobium loti K1 channel cyclic nucleotide-binding domain in complex with cAMP.,Schunke S, Stoldt M, Novak K, Kaupp UB, Willbold D EMBO Rep. 2009 Jul;10(7):729-35. Epub 2009 May 22. PMID:19465888[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Cooperative and uncooperative cyclic-nucleotide-gated ion channels.
Cukkemane et al. (2011)
2 more
24 other articles
No citations found

See Also

References

  1. ↑ Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH. Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel. Cell. 2004 Nov 24;119(5):615-27. PMID:15550244 doi:10.1016/j.cell.2004.10.030
  2. ↑ Schunke S, Stoldt M, Novak K, Kaupp UB, Willbold D. Solution structure of the Mesorhizobium loti K1 channel cyclic nucleotide-binding domain in complex with cAMP. EMBO Rep. 2009 Jul;10(7):729-35. Epub 2009 May 22. PMID:19465888 doi:10.1038/embor.2009.68

Contents


PDB ID 2k0g

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