Structural highlights
Function
FRA1E_FRAAN Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (PubMed:19969523). Binds the natural flavonoid quercetin-3-O-glucuronide with affinities in the low micromolar range (PubMed:24133217).[1] [2]
Publication Abstract from PubMed
The PR10 family protein Fra a 1E from strawberry (Fragaria x ananassa) is down-regulated in white strawberry mutants, and transient RNAi (RNA interference)-mediated silencing experiments confirmed that Fra a 1 is involved in fruit pigment synthesis. In the present study, we determined the solution structure of Fra a 1E. The protein fold is identical with that of other members of the PR10 protein family and consists of a seven-stranded antiparallel beta-sheet, two short V-shaped alpha-helices and a long C-terminal alpha-helix that encompass a hydrophobic pocket. Whereas Fra a 1E contains the glycine-rich loop that is highly conserved throughout the protein family, the volume of the hydrophobic pocket and the size of its entrance are much larger than expected. The three-dimensional structure may shed some light on its physiological function and may help to further understand the role of PR10 proteins in plants.
Solution structure of the strawberry allergen Fra a 1.,Seutter von Loetzen C, Schweimer K, Schwab W, Rosch P, Hartl-Spiegelhauer O Biosci Rep. 2012 Dec 1;32(6):567-75. doi: 10.1042/BSR20120058. PMID:22913709[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Munoz C, Hoffmann T, Escobar NM, Ludemann F, Botella MA, Valpuesta V, Schwab W. The strawberry fruit Fra a allergen functions in flavonoid biosynthesis. Mol Plant. 2010 Jan;3(1):113-24. doi: 10.1093/mp/ssp087. Epub 2009 Oct 29. PMID:19969523 doi:http://dx.doi.org/10.1093/mp/ssp087
- ↑ Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:http://dx.doi.org/10.1074/jbc.M113.501528
- ↑ Seutter von Loetzen C, Schweimer K, Schwab W, Rosch P, Hartl-Spiegelhauer O. Solution structure of the strawberry allergen Fra a 1. Biosci Rep. 2012 Dec 1;32(6):567-75. doi: 10.1042/BSR20120058. PMID:22913709 doi:http://dx.doi.org/10.1042/BSR20120058
