3a4k

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Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA and divalent cations at 2.17 angstrom resolution

Structural highlights

3a4k is a 14 chain structure with sequence from Haemophilus influenzae Rd KW20. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.17Å
Ligands:ACT, GOL, MG, MN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2D3_HAEIN Recognizes the double-stranded sequence AAGCTT and cleaves after A-1.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.

Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations.,Watanabe N, Takasaki Y, Sato C, Ando S, Tanaka I Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1326-33. doi:, 10.1107/S0907444909041134. Epub 2009 Nov 17. PMID:19966419[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
0 reviews cite this structure
Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonuclease.
Horton et al. (2012)
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2 other articles
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See Also

References

  1. ↑ Watanabe N, Takasaki Y, Sato C, Ando S, Tanaka I. Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations. Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1326-33. doi:, 10.1107/S0907444909041134. Epub 2009 Nov 17. PMID:19966419 doi:http://dx.doi.org/10.1107/S0907444909041134

Contents


PDB ID 3a4k

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