3d1n

From Proteopedia

Structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter

Structural highlights

3d1n is a 16 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.51Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PO6F1_HUMAN Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Brn-5 protein, highly expressed in human brain, belongs to the POU family; a class of transcription factors involved in a wide variety of biological processes ranging from programming of embryonic stem cells to cellular housekeeping. This functional diversity is conferred by two DNA-binding subdomains that can assume several configurations due to a bipartite arrangement of POU-specific (POU(S)) and POU-homeo (POU(H)) subdomains separated by a linker region. The crystal structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone (CRH) gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA. Moreover, the structure also shows the role of the linker in allowing diverse configurations that can be assumed by the two subdomains.

Structure of human Brn-5 transcription factor in complex with CRH gene promoter.,Pereira JH, Kim SH J Struct Biol. 2009 Aug;167(2):159-65. Epub 2009 May 18. PMID:19450691^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pereira JH, Kim SH. Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol. 2009 Aug;167(2):159-65. Epub 2009 May 18. PMID:19450691 doi:10.1016/j.jsb.2009.05.003