3g0h
From Proteopedia
Human dead-box RNA helicase DDX19, in complex with an ATP-analogue and RNA
Structural highlights
FunctionDD19B_HUMAN ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedDEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations. The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. Loading citation details.. Citations No citations found See AlsoReferences
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Categories: Homo sapiens | Large Structures | Arrowsmith CH | Berglund H | Bountra C | Collins R | Dahlgren LG | Edwards AM | Flodin S | Flores A | Graslund S | Hammarstrom M | Johansson A | Johansson I | Karlberg T | Kotenyova T | Lehtio L | Moche M | Nilsson ME | Nordlund P | Nyman T | Persson C | Sagemark J | Schuler H | Schutz P | Siponen MI | Thorsell AG | Tresaugues L | Van Den Berg S | Weigelt J | Welin M | Wisniewska M
