3i8t

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N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose

Structural highlights

3i8t is a 1 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:GAL, GLC, GOL, NA, PEG, PRD_900008
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LEG4_MOUSE Galectin that binds lactose and a related range of sugars (By similarity).

Publication Abstract from PubMed

Galectin-4, a member of the tandem-repeat subfamily of galectins, participates in cell-membrane interactions and plays an important role in cell adhesion and modulation of immunity and malignity. The oligosaccharide specificity of the mouse galectin-4 carbohydrate-recognition domains (CRDs) has been reported previously. In this work, the structure and binding properties of the N-terminal domain CRD1 were further investigated and the crystal structure of CRD1 in complex with lactose was determined at 2.1 A resolution. The lactose-binding affinity was characterized by fluorescence measurements and two lactose-binding sites were identified: a high-affinity site with a K(d) value in the micromolar range (K(d1) = 600 +/- 70 microM) and a low-affinity site with K(d2) = 28 +/- 10 mM.

Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition.,Krejcirikova V, Pachl P, Fabry M, Maly P, Rezacova P, Brynda J Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):204-11. Epub 2011, Feb 15. PMID:21358051[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Key regulators of galectin-glycan interactions.
Kamili et al. (2016)
2 more
5 other articles
No citations found

See Also

References

  1. ↑ Krejcirikova V, Pachl P, Fabry M, Maly P, Rezacova P, Brynda J. Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition. Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):204-11. Epub 2011, Feb 15. PMID:21358051 doi:10.1107/S0907444911004082

Contents


PDB ID 3i8t

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