3nir

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Crystal structure of small protein crambin at 0.48 A resolution

Structural highlights

3nir is a 1 chain structure with sequence from Crambe hispanica subsp. abyssinica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 0.48Å
Ligands:EOH
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRAM_CRAAB The function of this hydrophobic plant seed protein is not known.

Publication Abstract from PubMed

With the development of highly brilliant and extremely intense synchrotron X-ray sources, extreme high-resolution limits for biological samples are now becoming attainable. Here, a study is presented that sets the record in crystallographic resolution for a biological macromolecule. The structure of the small protein crambin was determined to 0.48 A resolution on the PETRA II ring before its conversion to a dedicated synchrotron-radiation source. The results reveal a wealth of details in electron density and demonstrate the possibilities that are potentially offered by a high-energy source. The question now arises as to what the true limits are in terms of what can be seen at such high resolution. From what can be extrapolated from the results using crystals of crambin, this limit would be at approximately 0.40 A, which approaches that for smaller compounds.

Crystal structure of small protein crambin at 0.48 A resolution.,Schmidt A, Teeter M, Weckert E, Lamzin VS Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt, 4):424-8. Epub 2011 Mar 24. PMID:21505232[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
5 reviews cite this structure
Tam et al. (2015)
No citations found

References

  1. Schmidt A, Teeter M, Weckert E, Lamzin VS. Crystal structure of small protein crambin at 0.48 A resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt, 4):424-8. Epub 2011 Mar 24. PMID:21505232 doi:10.1107/S1744309110052607

Contents


PDB ID 3nir

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