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Publication Abstract from PubMed

Glucokinase (GK) is a monomeric allosteric enzyme and plays a pivotal role in blood glucose homeostasis. GK is regulated by GK regulatory protein (GKRP), and indirectly by allosteric effectors of GKRP. Despite the critical roles of GK and GKRP, the molecular basis for the allosteric regulation mechanism of GK by GKRP remains unclear. We determined the crystal structure of Xenopus GK and GKRP complex in the presence of fructose-6-phosphate at 2.9 A. GKRP binds to a super-open conformation of GK mainly through hydrophobic interaction, inhibiting the GK activity by locking a small domain of GK. We demonstrate the molecular mechanism for the modulation of GK activity by allosteric effectors of GKRP. Importantly, GKRP releases GK in a sigmoidal manner in response to glucose concentration by restricting a structural rearrangement of the GK small domain via a single ion pair. We find that GKRP acts as an allosteric switch for GK in blood glucose control by the liver.

Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase.,Choi JM, Seo MH, Kyeong HH, Kim E, Kim HS Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10171-6. doi:, 10.1073/pnas.1300457110. Epub 2013 Jun 3. PMID:23733961^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.