3wxq
From Proteopedia
Serial femtosecond X-ray structure of human fatty acid-binding protein type-3 (FABP3) in complex with stearic acid (C18:0) determined using X-ray free-electron laser at SACLA
Structural highlights
FunctionFABPH_HUMAN FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Publication Abstract from PubMedSerial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 A. Grease matrix as a versatile carrier of proteins for serial crystallography.,Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S Nat Methods. 2014 Nov 10. doi: 10.1038/nmeth.3172. PMID:25384243[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. Loading citation details.. Citations No citations found See AlsoReferences
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Categories: Homo sapiens | Large Structures | Hatsui T | Iwata S | Joti Y | Kakinouchi K | Mizohata E | Murata M | Nango E | Song C | Sugahara M | Sugiyama S | Suzuki M | Tanaka R | Tanaka T | Tono K | Yabashi M
