| Structural highlights
Function
FABPH_HUMAN FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Publication Abstract from PubMed
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 A.
Grease matrix as a versatile carrier of proteins for serial crystallography.,Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S Nat Methods. 2014 Nov 10. doi: 10.1038/nmeth.3172. PMID:25384243[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S. Grease matrix as a versatile carrier of proteins for serial crystallography. Nat Methods. 2014 Nov 10. doi: 10.1038/nmeth.3172. PMID:25384243 doi:http://dx.doi.org/10.1038/nmeth.3172
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