4d74

From Proteopedia

1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI

Structural highlights

4d74 is a 1 chain structure with sequence from Erwinia amylovora ATCC 49946. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.57Å
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AMSI_ERWAM May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production.

Publication Abstract from PubMed

AmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 A resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism.

Characterization and 1.57 A resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora.,Salomone-Stagni M, Musiani F, Benini S Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):903-910. Epub 2016, Nov 30. PMID:27917839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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References

↑ Salomone-Stagni M, Musiani F, Benini S. Characterization and 1.57 A resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora. Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):903-910. Epub 2016, Nov 30. PMID:27917839 doi:http://dx.doi.org/10.1107/S2053230X16018781

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

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PDB ID 4d74

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4d74

From Proteopedia

1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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