4dau

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Structure of 14-3-3 sigma in complex with PADI6 14-3-3 binding motif I

Structural highlights

4dau is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:SEP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

1433S_HUMAN Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.

Publication Abstract from PubMed

The regulation and function of peptidylarginine deiminase isoform VI (PAD6), which is a highly abundant protein associated with the cytoplasmic lattices in mammalian oocytes, is poorly understood so far. It has been shown previously, that 14-3-3 proteins, a class of regulatory adapter proteins ubiquitous in eukaryotes, bind to PAD6 in vivo in a phosphorylation dependant manner. Here we identify possible 14-3-3 binding sites in human PAD6 by in silico methods, looking for conserved, surface exposed serine residues. Two of these sites were confirmed as 14-3-3 binding sites by fluorescence polarization competition and X-ray crystallography. We furthermore suggest a role of RSK-type kinases in the phosphorylation of one of these two binding sites and provide evidence in the form of in vitro kinase assays with p70S6 kinase and RSK1.

Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI.,Rose R, Rose M, Ottmann C J Struct Biol. 2012 May 24. PMID:22634725[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Modulation of Protein-Protein Interactions for the Development of Novel Therapeutics.
Petta et al. (2016)
2 more
16 other articles
No citations found

See Also

References

  1. ↑ Rose R, Rose M, Ottmann C. Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI. J Struct Biol. 2012 May 24. PMID:22634725 doi:10.1016/j.jsb.2012.05.010

Contents


PDB ID 4dau

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