4f4m
From Proteopedia
Structure of the type VI peptidoglycan amidase effector Tse1 (C30A) from Pseudomonas aeruginosa
Structural highlights
FunctionTSE1_PSEAE Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via amidase activity thereby helping itself to compete with other bacteria (PubMed:21776080, PubMed:22931054, PubMed:22813741, PubMed:22700987). To protect itself, the bacterium synthesizes immunity protein Tsi1 that specifically interacts with and inactivates cognate toxin (PubMed:21776080, PubMed:22931054, PubMed:22700987).[1] [2] [3] [4] Publication Abstract from PubMedThe target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity. Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.,Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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