4htj

From Proteopedia

Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin at pH 4.6

Structural highlights

4htj is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.011Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PTPR2_HUMAN Implicated in development of nervous system and pancreatic endocrine cells.^[1] ^[2]

Publication Abstract from PubMed

Phogrin/IA-2beta and ICA512/IA-2 are two paralogs receptor-type protein-tyrosine phosphatases (RPTP) that localize in secretory granules of various neuroendocrine cells. In pancreatic islet beta-cells, they participate in the regulation of insulin secretion, ensuring proper granulogenesis, and beta-cell proliferation. The role of their cytoplasmic tail has been partially unveiled, while that of their luminal region remains unclear. To advance the understanding of its structure-function relationship, the X-ray structure of the mature ectodomain of phogrin (ME phogrin) at pH 7.4 and 4.6 has been solved at 1.95- and 2.01-A resolution, respectively. Similarly to the ME of ICA512, ME phogrin adopts a ferredoxin-like fold: a sheet of four antiparallel beta-strands packed against two alpha-helices. Sequence conservation among vertebrates, plants and insects suggests that the structural similarity extends to all the receptor family. Crystallized ME phogrin is monomeric, in agreement with solution studies but in striking contrast with the behavior of homodimeric ME ICA512. The structural details that may cause the quaternary structure differences are analyzed. The results provide a basis for building models of the overall orientation and oligomerization state of the receptor in biological membranes.

X-ray structure of the mature ectodomain of phogrin.,Noguera ME, Primo ME, Jakoncic J, Poskus E, Solimena M, Ermacora MR J Struct Funct Genomics. 2015 Mar;16(1):1-9. doi: 10.1007/s10969-014-9191-0. Epub, 2014 Nov 26. PMID:25421040^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..

Citations

0 reviews cite this structure

A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis.

Katsura et al. (2017)

-1 more

4 other articles

No citations found

References

↑ Kawasaki E, Hutton JC, Eisenbarth GS. Molecular cloning and characterization of the human transmembrane protein tyrosine phosphatase homologue, phogrin, an autoantigen of type 1 diabetes. Biochem Biophys Res Commun. 1996 Oct 14;227(2):440-7. PMID:8878534 doi:S0006-291X(96)91526-0
↑ Cui L, Yu WP, DeAizpurua HJ, Schmidli RS, Pallen CJ. Cloning and characterization of islet cell antigen-related protein-tyrosine phosphatase (PTP), a novel receptor-like PTP and autoantigen in insulin-dependent diabetes. J Biol Chem. 1996 Oct 4;271(40):24817-23. PMID:8798755
↑ Noguera ME, Primo ME, Jakoncic J, Poskus E, Solimena M, Ermacora MR. X-ray structure of the mature ectodomain of phogrin. J Struct Funct Genomics. 2015 Mar;16(1):1-9. doi: 10.1007/s10969-014-9191-0. Epub, 2014 Nov 26. PMID:25421040 doi:http://dx.doi.org/10.1007/s10969-014-9191-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

loadScript /wiki/extensions/jsmol/j2s/core/package.js

loadScript /wiki/extensions/jsmol/j2s/core/corejmol.z.js

loadScript /wiki/extensions/jsmol/j2s/J/translation/PO.js

loadScript /wiki/extensions/jsmol/j2s/core/corescript.z.js

JSmol exec jmolApplet1 start applet null

Jmol JavaScript applet jmolApplet1__438524695614953__ initializing

Jmol getValue debug null

Jmol getValue logLevel null

Jmol getValue allowjavascript null

AppletRegistry.checkIn(jmolApplet1__438524695614953__)

vwrOptions:

{ "name":"jmolApplet1","applet":true,"documentBase":"https://proteopedia.org/wiki/index.php/4htj","platform":"J.awtjs2d.Platform","fullName":"jmolApplet1__438524695614953__","codePath":"https:/wiki/extensions/jsmol/j2s/","display":"jmolApplet1_canvas2d","loadStructCallback":"manageLoadStructCallback","signedApplet":"true","appletReadyCallback":"Jmol._readyCallback","statusListener":"[J.appletjs.Jmol object]","syncId":"438524695614953","messageCallback":"msgCb","bgcolor":"white" }

setting document base to "https://proteopedia.org/wiki/index.php/4htj"

Jmol Version: 14.31.8 2020-09-26 10:04

java.vendor: Java2Script (HTML5)

java.version: 2019-06-08 00:17:46 (JSmol/j2s)

os.name: Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)

Access: ALL

memory: 0.0/0.0

processors available: 1

useCommandThread: false

appletId:jmolApplet1 (signed)

Jmol getValue emulate null

defaults = "Jmol"

Jmol getValue boxbgcolor null

Jmol getValue bgcolor white

backgroundColor = "white"

Jmol getValue ANIMFRAMECallback null

Jmol getValue APPLETREADYCallback Jmol._readyCallback

APPLETREADYCallback = "Jmol._readyCallback"

StatusManager callback set for APPLETREADYCallback f=Jmol._readyCallback cb=APPLETREADY

Jmol getValue ATOMMOVEDCallback null

Jmol getValue AUDIOCallback null

Jmol getValue CLICKCallback null

Jmol getValue DRAGDROPCallback null

Jmol getValue ECHOCallback null

Jmol getValue ERRORCallback null

Jmol getValue EVALCallback null

Jmol getValue HOVERCallback null

Jmol getValue IMAGECallback null

Jmol getValue LOADSTRUCTCallback manageLoadStructCallback

LOADSTRUCTCallback = "manageLoadStructCallback"

StatusManager callback set for LOADSTRUCTCallback f=manageLoadStructCallback cb=LOADSTRUCT

Jmol getValue MEASURECallback null

Jmol getValue MESSAGECallback msgCb

MESSAGECallback = "msgCb"

StatusManager callback set for MESSAGECallback f=msgCb cb=MESSAGE

Jmol getValue MINIMIZATIONCallback null

Jmol getValue SERVICECallback null

Jmol getValue PICKCallback null

Jmol getValue RESIZECallback null

Jmol getValue SCRIPTCallback null

Jmol getValue SYNCCallback null

Jmol getValue STRUCTUREMODIFIEDCallback null

Jmol getValue doTranslate null

language=en_US

Jmol getValue doTranslate null

Note -- Presence of message callback disables disable translation; to enable message translation use jmolSetTranslation(true) prior to jmolApplet()

Note -- language translation disabled

Jmol getValue popupMenu null

Jmol getValue script null

Jmol getValue loadInline null

Jmol getValue load null

Jmol applet jmolApplet1__438524695614953__ ready

script 1 started

pdbGetHeader = true

FileManager opening url https://proteopedia.org/wiki/extensions/Proteopedia/spt/functions.spt?1733933141

loadScript /wiki/extensions/jsmol/j2s/core/coretext.z.js

Loading, please wait ...

platformSpeed = 8

loadScript /wiki/extensions/jsmol/j2s/core/corescriptmath.z.js

loadScript /wiki/extensions/jsmol/j2s/J/bspt/PointIterator.js

FileManager opening url https://proteopedia.org/cgi-bin/infopdb?4htj

loadScript /wiki/extensions/jsmol/j2s/JU/JSJSONParser.js

loadScript /wiki/extensions/jsmol/j2s/JU/JSONException.js

JSON parser cannot parse NetworkError

Data for 4htj could not be found.- Using defaults.

cache cleared

[Step 1] pdb_features.ca_p_only is: integer 0

Loading, please wait ...

PDB ID 4htj

Drag the structure with the mouse to rotate

Export Animated Image

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/4htj"

4htj

From Proteopedia

Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin at pH 4.6

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox