4i8c

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X-ray structure of NikA in complex with Ni-(L-His)2

Structural highlights

4i8c is a 3 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.503Å
Ligands:ACT, CL, GOL, HIS, NI, SO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NIKA_ECOLI Involved in a nickel transport system, probably represents the nickel binder.

Publication Abstract from PubMed

The ABC-type importer NikABCDE mediates nickel acquisition in Escherichia coli. The periplasmic nickel-binding component NikA has a folding similar to that of the peptide transporter OppA and does not bind free nickel. Instead, we showed that the metal is tetra-coordinated by an organic tri-dentate molecule and His416. Conversely, it has been recently reported that NikA binds Ni-((L)-His)(2) and that addition of histidine increases the rate of nickel uptake in vivo. Here, we report the structure of NikA/Ni-((L)-His)(2) and show that histidine binding differs from peptide binding in OppA. The structure also confirms the central role of His416 in nickel binding.

The binding mode of Ni-((L)-His)(2) in NikA revealed by X-ray crystallography.,Lebrette H, Iannello M, Fontecilla-Camps JC, Cavazza C J Inorg Biochem. 2012 Dec 23;121C:16-18. doi: 10.1016/j.jinorgbio.2012.12.010. PMID:23314594[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Common themes and unique proteins for the uptake and trafficking of nickel, a metal essential for the virulence of Helicobacter pylori.
de et al. (2013)
2 more
12 other articles
No citations found

See Also

References

  1. ↑ Lebrette H, Iannello M, Fontecilla-Camps JC, Cavazza C. The binding mode of Ni-((L)-His)(2) in NikA revealed by X-ray crystallography. J Inorg Biochem. 2012 Dec 23;121C:16-18. doi: 10.1016/j.jinorgbio.2012.12.010. PMID:23314594 doi:http://dx.doi.org/10.1016/j.jinorgbio.2012.12.010

Contents


PDB ID 4i8c

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