4j8a

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Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145

Structural highlights

4j8a is a 1 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.26Å
Ligands:CRO, EDO, HOX, SO4, TRS
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Publication Abstract from PubMed

Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.,Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry.
Reddington et al. (2013)
2 more
22 other articles
No citations found

See Also

References

  1. ↑ Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD. Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence. Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472 doi:10.1002/anie.201301490

Contents


PDB ID 4j8a

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OCA

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